Tutorial 3 – Extracellular Vesicles

Tuesday, 09.09.2025, 6:30 pm – 8:00 pm

Chair: Jochen Behrends

Abstract

Extracellular vesicles (EVs), a class of biological nanoparticles, are gaining attention in biomedical research for their roles in drug delivery, diagnostics, and regenerative medicine. EVs, naturally derived from cells, offer biocompatibility, low immunogenicity, and the ability to cross biological barriers, making them promising therapeutic candidates.

Flow cytometry is a powerful tool for EV detection and characterization, despite challenges related to their small size and heterogeneity. Advances in high-resolution flow cytometry, fluorescent labeling, and bead-based assays have improved EV analysis. This tutorial covers recent developments in nanoparticle technologies, focusing on EV detection via flow cytometry, with guidelines to address challenges and optimize methodologies.