Tutorials 4 and 5

Wednesday, 11.09.2024, 9:00 am

Tutorial 4: Mechanocytometry

Chairs: Daniel Klaue und Oliver Otto

In this tutorial, we will explore an innovative technique in cell characterization: real-time deformability cytometry (RT-DC). RT-DC is a label-free, high-speed (kHz) and versatile imaging method that combines mechanical, morphological, and optical parameters for real-time cell analysis.
A key highlight of this session will be the demonstration of our new fully automated blood analysis device, which employs RT-DC to automatically identify and classify various populations of blood cells, such as erythrocytes, neutrophils, monocytes, and more. This groundbreaking functionality allows for efficient and accurate blood cell analysis without the need for complex sample preparation or fluorescent markers.
We will also provide an opportunity for participants to interact with the data live, using our open-source analysis software, Shape-Out 2. We encourage you to install the software on your own laptop (available for download in our section) so that we can analyze and explore real RT-DC data together.
Additionally, we will showcase our established device, the AcCellerator, highlighting its powerful features in the context of mechanical and morphological cell analysis.
Join us for a hands-on session to see how RT-DC can revolutionize your research on blood cells and beyond!

Key Points:

  • Real-time deformability cytometry (RT-DC): Label-free, high-speed, versatile technique for cell imaging.
  • Blood Cell Analysis: Automatic identification and classification of blood cell populations (e.g., erythrocytes, neutrophils, monocytes).
  • Interactive Learning: Hands-on experience analyzing RT-DC data with Shape-Out 2.
  • Device Demonstration: Explore the AcCellerator’s capabilities for mechanical and morphological analysis.


Tutorial 5: High-Dimensional Cytometry: Choosing the Right Tool for Your Research

Marjolijn Hameetman1, Désirée Kunkel2, Tamar Tak1, Axel Ronald Schulz3

1 Flow Cytometry Core Facility, Leiden University Medical Center (LUMC), Leiden, Netherlands
2 BIH Cytometry Core Facility, Berlin Institute of Health (BIH) at Charite – Universitätsmedizin Berlin, Berlin, Germany
3 Mass Cytometry Lab, German Rheumatology Research Center, a Leibniz-Institute (DRFZ), Berlin, Germany

Abstract:
High-dimensional single-cell cytometry has revolutionized immunology and biomedical research, offering unparalleled insights into cellular complexity. In this interactive tutorial, we will introduce key technologies, with a focus on full spectrum flow cytometry and mass cytometry, comparing their capabilities, and highlighting advantages and limitations from a user’s perspective. Through practical examples, we will discuss factors such as panel design, resolution, costs and sample throughput to guide cytometrists in making informed choices. The session will conclude with an interactive exercise where attendees will assess real-world experimental scenarios and determine which technology to use for a specific research question and experimental setup. This hands-on approach will provide valuable insights