Chairs: Axel R Schulz, Claudia Peitzsch
Mass cytometry (Cytometry by time-of-flight, CyTOF) is one of the most powerful tools for high-dimensional single-cell analysis. Due to the lack of signal overlap and cellular autofluorescenceit enables simultaneous measurement of more than 50 parameters in cell suspensions and for spatial analysis of tissue sections. By conjugating antibodies to stable heavy metal isotopes and detecting them by time-of-flight mass spectrometry, the method offers excellent signal resolution, an extensive and expandable marker space, and a comparatively straightforward and rapid panel setup.
In this session, we will briefly introduce the newly formed Mass Cytometry Working Group, which became part of the DGfZ in January 2026. Our invited speaker Ezgi Senoglu from the CRTD in Dresden will present her work on Epi-CyTOF. She used this technique to study epigenetic regulation of human neocortical development in cortical organoids to gain a deeper understanding of the epigenetic deregulation in neuropathologies. In addition, we look forward to contributions from the mass cytometry community that will be selected for oral presentations.